Mix. Pineapple Enzyme Inquiry Lab. Using the background information and your knowledge of enzymes, write a hypothesis for this lab. Bromelain is an enzyme mixture present in the pineapple. and discussion/conclusion questions to answer. If papers are copied, the total number of points earned for the paper will be divided by the number of individuals involved in copying. Place Pineapple in the Microwave for 1:30 seconds 6. Repeat in the 3 other bowls except switch out the fresh pineapple with canned pineapple, meat tenderizer, and gelatin with no pineapple (1/2-1 teaspoon each). This lab activity had students to conduct various tests on enzymes in different environments to determine how the rate of reaction in decomposing hydrogen peroxide (H2O2) to water and oxygen gas was dependent on environmental adjustments. Label the two test tubes, one of which will contain the yeast mixture, with the other serving as the control. This conclusion was reached by averaging the all the trials seen within Table 1. 1414 Words. Question: Directions for Writing the Enzyme Lab Report General Directions 1. . Add the fruit into the different bowls. Unformatted text preview: Pineapple Enzymes and Gelatin I. These positive effects of bromelain activities towards these viruses have been shown in-vitro in our lab. Gelatin makes Jello a semi-solid. 1 January 2017. Pineapple CONCLUSION/DISCUSSION QUESTIONS: (things to think about and begin to answer now before you get to your lab report) 1. View Lab Report - Pineapple enzymes lab #1.docx from BIOL 100 at University of Nevada, Reno. Conclusion: 3 paragraphs 1. . Introduction Bromelain is a group of enzymes found in the fruit and stem of the pineapple plant.Pineapple is native to the Americas but is now grown throughout the world in tropical and subtropical regions. Introduction: Within all living organisms there are many chemical reactions taking place at the same time. Get 4 cubes of gelatin and place in petri dishes. The scope of this experiment includes Metabolic processes, such as cellular respiration, and it poisonous byproduct hydrogen peroxide. Stir well with a spoon until the gelatin is dissolved. The results of enzyme isolation from pineapple weevil obtained a pH of 7.0 with an enzyme activity of 1.081 units/g(13). Data: Experiments: Day 1: Observations; Day 2: Observations; Jell-O without Fruit. The results of the isolation of the bromelain enzyme from pineapple peel were obtained at a temperature of 65oC at 0.071 units/minute and a pH of 6.5 at 0.101 units/minute(12). . Write your conclusion. Pineapple Enzyme Lab: Vocab: In your science notebook, write the definitions of enzyme, active site, and substrate. Background: An enzyme in pineapple breaks down collagen which is a protein. The mitochondria within the cell will convert food into energy. Potato Enzyme Lab Report. . Put 2 pieces (about the same amount) of each type of pineapple different Cups. Gelatin comes from bones and cartilage from pigs and cows 3. Conclusion : I conclude that the enzyme bromelain brakes down the protein collagen found if gelatin, and that you need at least 5mL more enzyme than you do collagen for all of the collagen to be broken down in 24 hours. If this is true, bromelain may also be a gout-fighting enzyme that helps break up purines found in protein. The lab report must be typed with your name in the upper right hand corner and the date. Place one tablespoon of the pineapple into the . So this is was way to prove that enzymes might be a part of the juice. ENZYME LAB Background Information: Ask an expert. Pineapple Jelly Enzyme Lab. Step 1. Meat tenderizer contains a bromeliad. The major objective of this experiment was to observe the effects of catalase under varying controlled conditions. Before conducting the lab with students, I recommend to introduce students to enzymes first. What is the effect of heating? Similar to digestion, it softens meat by splitting up their protein bonds. Lab procedures should be conducted as stated in the Tropical Fruit Gelatin Lab attached. The methodology used was Experiment 7.2A: The effects of temperature on the rate of reaction of an enzyme, which was composed and developed by Oxford University Press Australia and New Zealand . Add one cube of the gelatin and one cube of fresh pineapple in one of the bowls. There were no pineapples, meaning no . Discussion 7. Each enzyme in a eukaryotic cells has specific molecules it binds to, as they have a specific frame work. The purpose of the lab, include vocabulary 2. Crush the pineapple in a blender until you have a fairly smooth but pulpy mixture. Conclusion The enzyme lab determined the function of enzymes on substrates, using pineapple and jello. Pineapple contains a digestive enzyme named bromelin. 3. 3. . Step 3. Enzymes are proteins that act as catalysts in living cells and carry out many of a cell's metabolic processes. Our hypothesis was that hydrogen peroxide would . In this test tube, the pineapple setted very quickly. Place 3 ml of the designated pineapple juice into each test tube. . Tube 4: gelatin + canned pineapple Tube 5: gelatin + fresh pineapple juice Tube 6: gelatin + juice from canned pineapple Fill in the data table after all tubes have chilled and answer the questions on the assignment sheet. Did you accept or reject your hypothesis? Make the gelatin according to the package directions. 434 Words; 2 Pages; Pineapple Jelly Enzyme Lab. 3. Purpose: Today we are going to use fresh, frozen, and canned pineapple to see how changes in temperature can affect bromelain's ability to digest the protein gelatin. Materials: 4 test . It is important to mention there are many different conclusions on the effects of bromelain. Bromelain was denatured 8/9 times in the 75 degree Celsius trial. There is another enzyme in the liver that helps break down the peroxide into water and. Bromelain is an enzyme that "promotes the digestive process and helps to break down proteins in foods" (Jomard, 2007). Vitamin C is a co-enzyme to Bromelain 5. It should be the ma addressed by the. Physics for Dummies reports that latent heat is the heat needed to cause a change in phase per kilogram. Jell-O & Pineapple Experiment by: Katrina Castro Background Information Purpose: In this lab we will determine how fast cooked pineapple vs. fresh pineapple can break down gelatin. Bromelain is an aqueous enzyme extract obtained from both the stem and fruit of the pineapple plant and it's the cause of why your tongue may become irritated or hurt when eating pineapple. Pineapple Enzyme Lab. State your QUESTION (Be sure it is written in the form of a question. Fill each of two test tubes 1/3 full with sucrose solution. The main ingredient responsible for this process is bromelain. It must be authorized by the teacher 4 Dixie cups Ice Tub Ice Stir sticks Procedure Get 4 Dixie cups and label each with tape * fresh * canned * no pineapple 2. We mistakenly assumed that because hot temperature would denature an enzyme, that the opposite would also apply. Hypothesis: The gelatin with cooked pineapple will break down slower than the gelatin with fresh The specific form of the protein sensitive to many different factors such as temperature, pH and other environmental conditions. . 3. They then started mixing the Jell-o powder into water to create the Jell-o 3. Failure to do so may result in mixing of the juice types and inaccurate results. Sources of Error 8. The bromelain successfully broke down the collagen proteins and not allowing them to unify. It's also remember that proteins are composed of amino acids that connect and fold into specific shapes to do a specific job. From your book or notes, what are all the factors that affect enzyme activity? Historically, natives of Central and South America used pineapple for a variety of ailments, such as digestive disorders. Materials and Equipment (Student) 4 test tubes, fresh pineapple, frozen pineapple, canned pineapple (chunky), powdered gelatin, . Pineapple Enzyme Lab: Vocab: In your science notebook, write the definitions of enzyme, active site, and substrate. Conditions in the environment around the enzyme (temperature, pH, ionic. We conducted the experiment so that we were able to have a control group utilizing water while we were able to change the variables with hydrogen peroxide, ammonia, and vinegar. Because of the enzyme that was in the pineapple, you could see a drastic change in the Jell-O itself, from it either being broken down completely, to not being broken down at all. The Lab Report 1. In this enzyme lab, you will use pineapple as an enzyme and Jell-O as a substrate to illustrate an enzyme/substrate complex. In this review General statements about your results and what they mean. Results 6. Keep the other bowl plain. . Materials: Four petri dishes Gelatin squares Fresh pineapple Frozen pineapple Canned pineapple Procedure: 1. Use a separate pipette for each type of juice. Label each section of report (with the exception of title). Pineapples have special enzymes that denature gelatin molecules. They speed up reactions by providing an alternative reaction pathway of lower activation energy. Allow the gelatin to cool and harden. Materials: Available for all: 4 small cups, hot gelatin, Temperature: one piece each of fresh pineapple, canned pineapple, frozen pineapple. Abstract 3. 4. Conclusion According to this lab it demonstrated that Jell-O does 348 Words; 2 Pages; Satisfactory Essays. Pour an equal amount of gelatin into each of the two bowls. 4. For the rest of the pineapples, the enzymes that prevent the jelly from becoming set are denatured, causing the jelly to harden. Then we poured cold rubbing alcohol into it, and almost immediately, the DNA became visible and stringy in the alcohol part of the solution. Accelerating chemical reactions is referred to as catalysis, and because enzymes take part in a chemical . Design an experiment to test the effects of temperature, pH, OR concentration on stopping the activity of bromelain, an enzyme found in pineapples. pH: vinegar, lemon juice, pH paper, 3 pieces of fresh . Take the Dixie cup to the gelatin and pour enough to cover the pineapple 4. Bromelain is a group of enzymes found in the fruit and stem of the pineapple plant. Part A: Determining the degree of enzymatic functioning in canned pineapple juice. . Label the dishes 1 - 4. After a short description of the uses of pineapple as folk medicine by the natives of the tropics, the more important new pharmaceutical applications of bromelain, reported between 1975 and 1978, are presented. 6 Pages. Bromelain helps relieve pain in arthritis. Fill beakers with the Jell-o 5. Failure to do so may result in mixing of the juice types and inaccurate results. . Conclusion: The active factors involved can be biochemically characterized only in part. We have learned that enzymes (proteins) in eukaryotic cells work as a catalyst to make this possible. Pineapple and jello enzyme lab answers Recall from the previous modules that enzymes are proteins that catalyze reactions. First gather materials. Also, the handouts will guide students in the process of writing a scientific lab report. 2. Enzyme Lab Report. Pineapple enzyme lab The most obvious learning objective for this lab is learning objective 4.17. Enzyme Pineapple Lab BACKGROUND: Gelatin is made from a protein called collagen, which comes from the joints of animals. Bromelain, the enzyme complex of pineapple (Ananas comosus) and its clinical application. Add 3 ml yeast suspension to one tube. Bromelain is an enzyme that "promotes the digestive process and helps to break down proteins in foods" (Jomard, 2007). In another report, several acetylcholinesterase (AChE) inhibitors, including tacrine. Conclusion- Our hypothesis was correct, the piece of gelatin continually got smaller. Divide the gelatin among the bowls. The guided procedure will allow you to discover that the processing of food, specifically canned pineapple, will denature the enzymes and render them useless. The Enzyme Lactase and Lactose Intolerance Lab. Procedure: Boil 100 mL of distilled water. Add ten small chunks of fresh pineapple to one bowl. Jell-O with cooked Pineapple. Feb-Mar 1988 . JELLO ENZYME LAB HAVE YOU EVER NOTICE THE WARNING ON A JELLO PACKAGE NOT TO USE FRESH OR FROZEN PINEAPPLE? The prediction was partly correct. Read More. Add the gelatin slowly to the boiling water and use a stir rod to continuously mix the gelatin in with the water. The chemical that the enzyme acts upon is the substrate. 2. Ms. Balmer Pineapple Jelly Enzyme Lab Discussion After completing the Pineapple Jell-O Enzyme lab, the final results were that the . Proteins in Pineapple Hypothesis- We think that pineapple juice will have protein in it because enzymes are made of protein and I believe that pineapple juice has enzymes in it. General statements about your results and what they mean. Fresh pineapple 2 bowls Measuring cup Kettle Water Spoon Two quarters 2 containers of gelatin Procedure: Mix the gelatin powder with warm or hot water according to the package's instructions. [1] The law of conservation of energy states that energy is neither created nor destroyed. If you have ever made Jell-O by cooking the powder that comes in a box, you may . Use a separate spoon for each type of juice. coming to the conclusion that heat does have an effe and will give us less product. 6. Set Timer 8. Background: If you have ever made Jell-O by cooking the powder that comes in a box, you may have noticed the warning on the instructions that tell you not to add fresh or frozen pineapple to the gelatin. Conclusion: In this conducted experiment, the variable measured was the duration of the reaction on a raw sweet potato. 8. Preparation phase/Pre-lab. STEPS. We did ours with pineapple, orange, strawberry and kiwi. TODAY WE ARE GOING TO INVESTIGATE WHY. 6. Enzymes are proteins that speed up chemical reactions, and are found in all living organisms because they are important in every day functions like digestion and blood clotting. Refrigerate for a few hours to set. Test the sucrose solution with a glucose test strip for the presence of glucose. What conclusions can you make about fresh versus canned juice? Bromelain is an aqueous enzyme extract obtained from both the stem and fruit of the pineapple plant and it's the cause of why your tongue may become irritated or hurt when eating pineapple. Bromelain, the enzyme complex of pineapple (Ananas . People tend to use bromelain as a supplement for various health benefits, including relieving sinus problems, reducing inflammation, and improving . 2. This product includes: Student Worksheets (6 pages) Teacher answer key ; Pictures of actual lab and results; This product will cover the following topics: Enzyme . and discussion/conclusion questions to answer. The original experiment had milk set to three different temperatures; Less than 15 o C, 37 o C and 60 o C. Each had a set time of 10 minutes to allow . . Materials and Methods 5. Historically, natives of Central and South America used pineapple for a variety of ailments, such as digestive disorders. a. Our data does not match our hypothesis because we predicted that because of denaturing, which is defined as the breaking down of proteins by heat, acidity, etc, the pineapple enzyme bromelain would become inactive. 7. Introduction 4. Question: How do pineapple enzymes of differing conditions (raw, cooked, and frozen) effect gelatin? The lab report must be written individually. PAP PINEAPPLE ENZYMES & JELLO LAB. Let stand for 20 minutes. It also breaks "collagen, preventing Jell-O from gelling . Conclusion Summarize the overall results of the lab and state the general scientific conclusions in regards to how purity, temperature and pH of pineapple juice affected the enzyme activity. Comment on your hypothesis, sources of error Gelatin Cup 1: Water b. Demo 2: Liver Catalase Demonstration Catalase is an enzyme that converts hydrogen peroxide (produced naturally by Pineapple plants produce this enzyme as a self-defence mechanism. The chemical properties of fresh pineapple are that it contains bromelain, vitamin C, antioxidants, carbohydrates and helps defend the immune system. Jell-O with fresh Pineapple. Materials: 4 test . Lab Report - Activity 5: Catalase Enzyme Activity. then added a pinch of salt, liquid detergent and pineapple juice, which was the enzyme the enzyme , to the Gatorade ( lysis . Introduction We have studied information about chemical reactions and how a catalyst is used to speed up reactions. PINEAPPLE AND. Good Essays. B. First we swished Gatorade in our mouths to get cheek cells (homogenization), then added a pinch of salt, liquid detergent and pineapple juice, which was the enzyme the enzyme, to the Gatorade (lysis). Gelatin may be dissolved in hot water. Place Pineapple in the Jell-o at the same time 7. . They also act like scissors and cut bonds between molecules. Set a timer for 20 minutes and watch your experiment. another learning objective is learning objective 4.3. Pineapple Enzyme Lab: Vocab: In your science notebook, write the definitions of enzyme, active site, and substrate. Lab procedures should be conducted as stated in the Tropical Fruit Gelatin Lab attached. 1. Hypothesis: We think that the fresh pineapples and the frozen fresh pineapples will prevent the jelly from becoming set. What is the effect of changing the pH? The purpose of this experiment is to determine the latent heat fusion of ice. . 4. View full document See Page 1 Conclusion: After the experiment, it was concluded that the cooked pineapple and canned pineapple can be used with Jell-O so that it remains solid; fresh pineapple and frozen pineapple will leave the Jell-O in a liquid form. 1845 2. These reactions are done so the food we eat can be small enough to fit into cells. They start to pour the Ice in the Tub 4. Take fresh-cut pineapple and crush it finely. You can heat it up to 67C and the enzyme will be active, . Conclusion: The active factors involved can be biochemically characterized only in part. Comment on your hypothesis, sources of error Title 2. Pineapple belongs to a group of plants called Bromeliads. Do not place anything but gelatin in dish 1. Conclusion: 3 paragraphs 1. Student will demonstrate understanding of concepts by completing end of lesson quizzes via https://www.pearsonrealize.c om. This is a fun and easy experiment that demonstrated the properties of enzymes. Pour 5 mL of gelatin into 5 test tubes. It also breaks "collagen, preventing Jell-O from gelling . Place gelatin and hot water into 250mL jug and mix with stirring rod until gelatin has dissolved. The purpose of this lab was to show the enzyme substrate concentration rate based on a varied enzyme and constant substrate. Observations of the reactions were recorded after twenty-five minutes and twenty-four hours. BACKGROUND. I made 5 separate ones using petri dishes. Pineapple contains a digestive enzyme named bromelin. Recombinant DNA Lab Conclusion January 13, 2016 Get link; Facebook; Twitter; Pinterest; Email; Other Apps; To produce recombinant DNA (rDNA), you first need to isolate, then get a a restriction enzyme to cut the . Open Document. Bromelain enzyme originating from the flesh and the stem of pineapple fruit has been shown to have the potential to be utilized as antiviral agent againstviral borne diseases which include HIV/AIDS, Hepatitis C and HPV that could cause . Enzymes and gelatin. 3. Pineapple Enzyme Formal Lab Report Lab 5 Pineapple Enzymes Formal Lab Report Guidelines General: Size 12 font, Times New Roman, 1 inch margins, double spaced. Cup 2: Fresh pineapple juice c. Cup 3: Canned pineapple juice d. Cup 4: Frozen pineapple juice (thawed) 5. The chemical properties of fresh pineapple are that it contains bromelain, vitamin C, antioxidants, carbohydrates and helps defend the immune system. The experimenter placed a chunk of fresh and canned pineapple on two separate petri dishes with jello. IV. The purpose of the lab, include vocabulary 2. The major change that would be necessary to make is the measurement each pineapple piece. pineapple jello lab conclusion that the enzymes in the pineapple will denature when the temperature is changed. An update J Ethnopharmacol. The methodology includes procedures; multiple variables were . Controls are essential in a lab to help rule out any unnecessary results. You need a fresh pineapple (canned with not work) with the skin removed, three bowls, and a box of Jello gelatin. . . Bromelain, the enzyme complex of pineapple (Ananas . Place 3 ml (approximately one spoon full) of the designated pineapple juice into each cup. In conclusion heating pineapple juice does affect the enzyme bromelain. Step 2. Double the Substrate Hypothesis- We think that if we make twice the amount of substrate accessible for the enzyme, it will break it down twice as fast. for the following lab activity conclusions: Lab - Testing for Identifying Organic Compounds, Lab- pH and Enzyme Action, Lab - "What's in My Lunch", and Pineapple Enzyme Lab. The sap of the pineapple plant contains much higher concentrations of bromelain and can cause severe pain if eaten. test tube 3, resulted, as expected, in the gelatin mixture not forming a solid. Like all catalysts, enzymes take part in the reaction - that is how they provide an alternative reaction pathway. Then we left one without fruit as the control. Conclusion- There was protein in the pineapple juice, and if there wasn t then our hypothesis about enzymes would be incorrect. Enzyme Bromelain is in Pineapple 4. Materials and Equipment (Student) 4 test tubes, fresh pineapple, frozen pineapple, canned pineapple (chunky), powdered gelatin, . Pour equal amounts into 12 labelled petri dishes and place in the cool room to set for a day. Enzymes in Pineapple Background: Enzymes are very efficient catalysts for biochemical reactions. The group first started by cutting out equal-sized pineapples. If this lab were repeated, the size of the pineapple piece would have to be regulated to ensure the same . Measure out 4 grams of gelatin. [2] Instead, energy is transferred; the first Law of Thermodynamics is . Part. In a typical enzyme- catalyzed reaction, the enzyme will bind briefly with the substrate, allowing the substrate to form its products. Pineapple is native to the Americas but is now grown throughout the world in tropical and subtropical regions.